Thin-layer chromatographic analysis of steroids

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USA Home > Product Directory > Analytical/Chromatography > LPLC/TLC/Paper Chromatography > Thin Layer Chromatography (TLC) > TLC and HPTLC Plates

Since different compounds will travel a different distance in the stationary phase, chromatography can in effect be used as an isolation technique. The separated compounds each occupying a specific area on the plate, they can be scraped away, put in another solvent to separate them from the stationary phase and used for further analysis. As an example, in the chromatography of an extract of green leaves (for example spinach ) in 7 stages of development, Carotene elutes quickly and is only visible until step 2. Chlorophyll A and B are halfway in the final step and lutein the first compound staining yellow. Once the chromatography is over, the carotene can be removed from the plate, put back into a solvent and ran into a spectrophotometer to characterize its wavelength absorption.

Thin-layer chromatographic analysis of steroids

thin-layer chromatographic analysis of steroids

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